As a continuation, the chemical constituents present in ethyl acetate extract of calotropis gigantea l. Studies on antidiarrheal activity of calotropis gigantea. But the 200 mg kg dose of ethanolic extract calotropis gigantea showed maximum anti inflammatory effect and was comparable to that produced by 100mg kg of. Pdf wound healing activity of latex of calotropis gigantea. Preliminary phytochemical screening and gc ms issn profiling. Pdf antidiabetic activity of calotropis gigantea in. Antifungal, antioxidant and antibacterial activities of. The nematodes meloidogyne incognita and meloidogyne javanica are found on the roots of calotropis gigantea in india, although the leaf extract kills them. Pdf the antimicrobial activity of crude aqueous extract of latex of calotropis gigantea apocynaceae was tested against six pathogenic species of. Ramesh chandra bundelkhand university, jhansi, uttar pradesh, india.
Giganteol which was first obtained from the stem bark is one of them and a new triterpene alcohol, isogiganteol is the other. It is widely used in many ayurvedic treatments, both externally ksharasutra and internally. Evaluation of antibacterial activity of calotropis gigentica against streptococcus mutans and lactobacillus acidophilus. Singh n, jain nk, kannojia p, garud n, pathak ak, mehta sc, in vitro antioxidant activity of calotropis gigantea hydroalcohlic leaves extract. Abstractcalotropis procera and calotropis gigantea are medicinal. Br leaves eecg was investigated in pylorus ligation and ethanol induced ulcer models in experimental rats. The hypoglycemic activity in normal rats was carried out by treatment using chloroform. The chloroform extracted fraction of latex showed inhibitory effect against s.
In ovalbumin induced asthma, rats were sensitized and challenged with ovalbumin ova. Both of methanol extract and its chloroform fraction showed activity against sarcina lutea, bacillus megaterium and pseudomonas aeruginosa. Plants profile for calotropis gigantea giant milkweed. Calotropis gigantea linn apocynaceae is a glabrous or hoary, laticiferous shrubs or small trees, about 34 m tall commonly known as the swallowwort or milkweed. Antifungal, antioxidant and antibacterial activities of calotropis procera mushtaq ahmed, rehmat ali khan, s.
Aiton is a spreading shrub or mediumsized tree reaching 2. Calotropis gigantea is an important indian medicinal plant and widely used in ayurveda for management of various diseases. The 11 analysis of antimicrobial activity of aqueous, methanolic and ethanolic extract of leaves and 12 flower of calotropis procera was carried out in disc method and also determined mic value at 600nm through optical density using spectrophotometer. This page was last edited on 15 december 2019, at 08.
The results of anti inflammatory effect of calotropis gigantea are showed in table 2. Calotropis species recorded for a number of activities like. The latex of the plant is also reported to possess analgesic and wound healing activity 19, 20, as well as antiinflammatory 21 and antimicrobial activities 22. Antimicrobial activity of latex of calotropis gigantea against pathogenic. Inhibitory effect of calotropis gigantea extract on ovalbumin. The ph of latex of these two species has been found different in the present study. Chitme institute of pharmacy, bundelkhand university, jhansi, uttar pradesh, india ramesh chandra bundelkhand university, jhansi, uttar pradesh, india sadhna kaushik department of pharmacology, m. The aim of the present study was to screen leaves of calotropis gigantea for the antimicrobial activity against clinical isolates of bacteria.
Fresh calotropis gigentica leaves were collected from the wasteland of jaipur and after washing shade dried at room temperature for 34 days. The leaves are sessile and subsessile, opposite, ovate, cordate at the base. The extraction was performed for 24 h with methanol. Preliminary phytochemical screening of calotropis gigantea. The latex of calotropis gigantea contains cardio glycosides, volatile fatty acids and calcium oxalate. Calotropis gigantea is a common wasteland weed and known for various medicinal properties. Phytochemical analysis, antibacterial and antioxidant activity of.
All structured data from the file and property namespaces is available under the creative commons cc0 license. Provided by smithsonian institution, department of botany. The leaves are broadly elliptical to oblongobovate in shape, with the size of 9. Antimicrobial activity of latex silver nanoparticles. Present study was undertaken to investigate the effect of methanolic extract of root of calotropis gigantea linn. Sep 22, 2015 the sodom apple calotropis gigantea, a member of the asclepiadaceae family, is a large evergreen shrub native to continental asia and northern africa. Pdf antimicrobial activity of akanda calotropis gigantea l. Result of the antimicrobial activity of root extracts of calotropis gigantea. The extracts were filtered with whatman filter paper to obtain a clear.
Antidiabetic activity of ethanol extract of cassia klenii leaf in streptozotocininduced diabetic rats and isolation of an active fraction and toxicity evaluation of the extract. Chitme 1 institute of pharmacy, bundelkhand university, jhansi, uttar pradesh, india. Antimicrobial activity and phytochemical analysis of calotropis gigantea root, latex extracts. Calotropis procera is a species of flowering plant in the family apocynaceae that is native to north africa, tropical africa, western asia, south asia, and indochina. Efficacy of antibacterial activity of calotropis gigantea. The antibacterial activity of methanol extract from the root bark of akanda calotropis gigantea l. Cg on ovalbumin induced asthma and arachidonic acid induced paw edema in rats. It is a very common herb seen widely throughout india.
Asclepiadaceae, commonly known as milkweed or swallowwort, is a common wasteland weed singh et al. Pdf the antibacterial activity of methanol extract from the root bark of akanda calotropis gigantea l. Venkatesan saratha and sorimuthu pillai subramanian department of biochemistry, university of madras guindy campus, chennai, tamil nadu, india. An recent approach on calotropis gigantea and calotropis procera. International research journal of pharmaceutical and. Chemical composition of calotropis gigantea springerlink. In both models the common parameter determined was ulcer index. Antimicrobial activities of calotropis procera on selected. A downloadable pdf document of a book in prepublication awaiting illustration.
Pharmpcology, nri institute of pharmaceutical sciencesnips, bhopal 2asst professor, nips, bhopalmp krishna. Dec 03, 2010 to evaluate the hypoglycemic and antidiabetic activity of chloroform extract of calotropis gigantea leaves and flowers in normal rats and streptozotocin induced diabetes. Pdf antimicrobial activity of latex of calotropis gigantea against. Calotropis gigantea white asclepiadaceae, is a weed plant commonly known as giant milkweed.
Asclepiadaceae a wildly growing plant has been reported to possess number of medicinal properties and other purposes. It is a body reaction in order to eliminate or limit the spread of injurious agent as. The root and latex of calotropis gigantea were screened for its antimicrobial and phytochemical activities. Extracts on growth and survival dynamics of selected pathogenic microorganisms the growth profiles of c. Transcriptomic and metabolic responses of calotropis. Calotropis gigantea latex, antifungal activity, minimum fungicidal concentration, zone of inhibition, disc diffusion method correspondence to author. Cns activity of calotropis gigantea roots sciencedirect. The green fruits contain a toxic milky sap that is extremely bitter and turns into a gluey coating which is resistant to soap. The extractions from latex are quit tricky in context with preparation of latex for the extraction purpose.
Invitro studies on calotropis procera leaf extracts as inhibitors of. Calotropis procera, latex, antimicrobial, antioxidant, antifungal. Different biochemical screening have been carried out to identify the important phyto. International journal of scientific and research publications, volume 4, issue 2, february 2014 1 issn 22503153. Each flower consists of five pointed petals and a small, elegant crown rising from the centre, which holds the stamens. General information crown flower is a fastgrowing, attractive, evergreen flowering shrub or small tree that grows about 5 metres tall, occasionally to 10 metres. Calotropis gigantea and calotropis procera are to a great extent having a very similar chemical properties, but the colours of their flowers are different.
The aim of our present study is to evaluate the antidiabetic activity of proteases extracted from specific protein antigen, bovine serum albumin bsa using calotropis gigantea, flowers dissolved. Calotropis procera is a well known plant and has been traditionally used for diarrhea, stomacic, sinus, fistula, and skin disease, alikhan and khanum 2005, raghubir et al. Small trees or shrubs, these two species are native to southern asia and africa and were introduced to south america and the islands of the caribbean, where they have naturalized. Studies on antidiarrhoeal activity of calotropis gigantea. It has a deep taproot, 34 m deep, and a secondary root system with woody lateral roots that may rapidly regenerate adventitious shoots when the plant is injured. Batch extraction the objective of the batch experiments is to measure the global kinetics of extraction and to investigate the influences of the operating parameters. Antimicrobial activity of akanda calotropis gigantea l. An excellent, if rather terse, guide to the traditional medicinal uses of the plants of the region in nepal, the latex is applied to sprains, body pains, boils and pimples. I am a research scholar and want to collect flowers of calotropis gigantea flowers for my study. The plant is erect, tall, large, much branched and perennial with milky latex throughout. Introduction inflammation is defined as the local response of living mammalian to injury due to agent. The agar diffusion test was used to check the antimicrobial activity of the.
The paw licking time was delayed and the numbers of writhings were greatly reduced. Antimicrobial activity and phytochemical analysis of. Name of species calotropis gigantea common name milkweed, swet ark family apocynaceae photo taken on 12 july, 2008 at sadashiv jyotirling, kukas, jaipur, rajasthan description dry leaf powder used for treating wounds and boils. An in vitro study venkatesan saratha and sorimuthu pillai subramanian department of biochemistry, university of madras guindy campus, chennai, tamil nadu, india abstract medicinal plants are important source of potentially useful. Mudarol, the chief resinol component of the root bark ofcalotropis gigantea, has been separated into two isomeric compounds by fractional crystallisation of the acetates and also by chromatographic adsorption analysis of the free alcohols.
The search for novel antibiotics like compounds from medicinal plants. Preliminary phytochemical screening of calotropis gigantea leaf suchita singh, sanchita singh, r. Studies on antidiarrhoeal activity of calotropis gigantea r. Study on antibacterial activity of calotropis procera. The antimicrobial activity of crude aqueous extract of latex of calotropis gigantea apocynaceae was tested against six pathogenic species of bacteria staphylococcus aureus, bacillus cereus, escherichia coli, micrococcus luteus, klebsiella pneumoniae and pseudomonas aeruginosa and two species of fungi candida krusei and aspergillus niger. Calotropis gigantea crown flower is a species of calotropis native to. To evaluate antimicrobial and phytochemical activity of hexane and ethanolic extract of. Table 1 showed preliminary phytochemical screening of plant parts of calotropis gigantea. Jun 15, 2006 alcoholic extract of peeled roots of calotropis gigantea r. The study was approved by the institutional animal ethical committee of b. Chemical characterization and insecticidal activity of. Preliminary phytochemical screening of calotropis gigantea leaf.
Sadhna kaushik department of pharmacology, maharani laxmi bai medical college, jhansi, uttar pradesh, india. The first species being abundant while the second being restricted to forest areas. It has one of the important traditional medicines to treat. Inhibitory effect of calotropis gigantea extract on. Chitme hr, chandra r, kaushik s, evaluation of antipyretic activity of calotropis gigantea asclepiadaceae in experimental animals.
The sodom apple calotropis gigantea, a member of the asclepiadaceae family, is a large evergreen shrub native to continental asia and northern africa. Preliminary phytochemical screening and gc ms issn. Leaves and flowers in streptozotocininduced diabetic rats. Previous studies on the antimicrobial activity of c. The purpose of the present study was to evaluate scientifically the antidiarrhoeal effects of c. The leaves are broadly elliptical to oblongobovate in shape, with the size of 920 cm x 612. Ocimum sanctum and calotropis gigantea leaves on clinical pathogens. Asclepiadaceae was tested orally in albino rats at the dose level of 250 and 500 mgkg bodyweight for cns activity. Pdf antimicrobial properties of ocimum sanctum and. Other articles where calotropis gigantea is discussed. Calotropis gigantea is a well known medicinal herb commonly known as madar has been used in unani, ayurveda, and siddha system of medicine for years. The antimicrobial effect of ethanol, aqueous and chloroform extracts of leaf and latex of calotropis procera on six bacteria namely, escherichia coli. Three hundred grams of powdered calotropis was separately macerated with 100% ethanol.
Phytochemical investigation of different plant parts of. Evaluation of antibacterial activity of calotropis. Studies on antidiarrheal activity of calotropis gigantea r. Evaluation of antibacterial activity of calotropis gigantea latex extr. Pdf antidiabetic activity of calotropis gigantea in human. Ethanolic extract of calotropis gigantea at a dose of 150 and 300mgkg produced significant inhibition of the gastric lesions induced by pylorus.
The plant has oval, light green leaves and milky stem. Evaluation of antibacterial activity of calotropis gigentica. Calotropis procera is a wild plant species in the family apocynaceae that is able to grow in harsh, arid and heat stressed conditions. Understanding how this highly adapted plant persists in harsh environments should inform future efforts to improve the hardiness of crop and forage plant species.
Files are available under licenses specified on their description page. Studies on endophytic fungi of calotropis procera l. Antioxidant, antimicrobial activities and gcms analysis of. Evaluation of antibacterial activity of calotropis gigentica against. The present study was carried out to evaluate the antioxidant and antibacterial activity of leaves extracts of calotropis procera and calotropis gigantea. Prominent analgesic activity was observed in eddys hot plate method and acetic acid induced writhings. Extracts on growthand survival dynamics of selected pathogenic microorganisms david m1, bharath k r2, bhavani m3 1,3department of biotechnology, sir padampat singhania university, udaipur, 3601, india. Significant activity was shown by calotropis gigantea doses as compared to control. As an important medicinal shrub and a fiber resource plant, there is an urgent need for developing molecular markers to facilitate breeding and genetic improvement of varieties. The antiulcer activity of ethanolic extract of calotropis gigantea r.
Can you please tell me the place from where i can get them in large quantity so that my work can be easily started. Calotropis gigantea is one such plant found widespread in most of the agricultural and nonagricultural fields. Wound healing activity of latex of c alotropis gigantea article pdf available in international journal of pharmacy and pharmaceutical sciences 11. Review of literature ayurvedic system of medicine is widely practised and accepted by an estimated 65% of the population in rural areas in india. Shahzaib, abdullah khan, arif waqar zaif, wasim ahmed department of biotechnology, university of science and technology bannu, khyber pakhtunkhwa pakistan key words. The ayurveda medicine system and medicinal plants help to meet their primary health care needs farnsworth et al. In this study, using the illumina high throughput sequencing. Pdf many drugs commonly used today are of herbal origin.
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